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| 名称 | HS-27A (人骨髓细胞) (STR鉴定正确) |
| 别称 | HS27A; HS27a |
| 种属 | 人类 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | HS-27A is a fibroblast cell line that was isolated in (year from History) from the stroma of a 30-year-old White male donor. HS-27A is a subclone of HS-27.HS-27A forms large flattened polygonal shaped cells that exemplify "blanket" cells and maintain numerous intercellular contacts with neighboring cells. Unlike HS-5, HS-27A secretes low levels of growth factors and does not support proliferation of isolated progenitor cells in cocultures. HS-27A expresses relatively high levels of vascular cell adhesion molecule 1 (VCAM-1). |
| 年龄（性别） | 男性；30岁 |
| 组织来源 | 骨头；骨髓；基质 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
| 倍增时间 | ~36h-48 hours |
| 抗原表达情况 | Vascular cell adhesion molecule 1 (CD106, VCAM-1) |
| 应用领域 | 3D cell culture； Immunology |
| 保藏机构 | ATCC; CRL-2496 |
