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产品概述
Ham's F-12营养混合物(Ham's F-12 Nutrient Mixture)是由Ham在1969年以Ham's F-10营养混合物为基础设计而成的,最初用于CHO细胞的无血清培养。Ham's F-12常作为无血清培养的基础培养液,在低血清含量下时,特别适合单细胞培养和克隆化培养,添加血清后也广泛应用于癌细胞和原代细胞的培养,如大鼠肝细胞、大鼠前列腺上皮细胞、软骨细胞、大鼠成肌细胞、鸡胚胎细胞等。此外,Ham's F-12与DMEM等体积混合后,得到的DMEM/F12培养基营养更为丰富,使用更为广泛。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
PH | 7.2~7.4 |
L-谷氨酰胺 | 1mM |
NaHCO3 | 1176mg/L |
D-葡萄糖 | 1802mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 1.2mg/L |
储存条件 | 2~8℃,避光 |
运输条件 | 常温 |
有效期 | 12个月 |
注意事项
1、本产品经过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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TEAD4 functions as a prognostic biomarker and triggers EMT via PI3K/AKT pathway in bladder cancer(2022/05/17)
作者:Ming Chi, Jiao Liu, Chenxue Mei
期刊:JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI:10.1186/s13046-022-02377-3
影响因子 :12.658
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The alternation of halobenzoquinone disinfection byproduct on toxicogenomics of DNA damage and repair in uroepithelial cells(2023/12/22)
作者:Xu Zhang, Lifang Liu, Jun Wang
期刊:ENVIRONMENT INTERNATIONAL
DOI:10.1016/j.envint.2023.108407
影响因子 :11.800
引用产品:PM150810
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A novel 450-nm laser-mediated sinoporphyrin sodium-based photodynamic therapy induces autophagic cell death in gastric cancer through regulation of the ROS/PI3K/Akt/mTOR signaling pathway(2022/12/08)
作者:Xing Li, Lijiang Gu, Yuhang Chen
期刊:BMC Medicine
DOI:10.1186/s12916-022-02676-8
影响因子 :9.300
引用产品:PM150810
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Dual PI3K/mTOR inhibitor PF-04979064 regulates tumor growth in gastric cancer and enhances drug sensitivity of gastric cancer cells to 5-FU(2023/12/29)
作者:Ziyuan Zhong, Tengkai Wang, Ruochen Zang
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2023.116086
影响因子 :7.500
引用产品:PM150810
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Quercetin induces ferroptosis in gastric cancer cells by targeting SLC1A5 and regulating the p-Camk2/p-DRP1 and NRF2/GPX4 Axes(2024/01/06)
作者:Lixian Ding, Shuwei Dang, Mingjun Sun
期刊:FREE RADICAL BIOLOGY AND MEDICINE
DOI:10.1016/j.freeradbiomed.2024.01.002
影响因子 :7.400
引用产品:PM150810
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Synergistic protection of quercetin and lycopene against oxidative stress via SIRT1-Nox4-ROS axis in HUVEC cells(2022/10/18)
作者:Xuan Chen, Liufeng Zheng, Bing Zhang
期刊:Current Research in Food Science
DOI:10.1016/j.crfs.2022.10.018
影响因子 :6.300
引用产品:PM150810
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KIRREL promotes the proliferation of gastric cancer cells and angiogenesis through the PI3K/AKT/mTOR pathway(2023/11/01)
作者:Tao Wang, Shuo Chen, Ziliang Wang
期刊:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子 :5.300
引用产品:PM150810
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Cancer-associated fibroblast expression of glutamine fructose-6-phosphate aminotransferase 2 (GFPT2) is a prognostic marker in gastric cancer(2023/07/03)
作者:Shuo Yang, Guoli Li, Xin Yin
期刊:Journal of Pathology Clinical Research
DOI:10.1002/cjp2.333
影响因子 :4.100
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Irradiation Induces Gasdermin E-Triggered Tumor Immunity to Inhibit Esophageal Carcinoma Cell Survival(2023/11/29)
作者:Bingxu Tan, Nana Wang, Shengsi Yang
期刊:ACS Omega
影响因子 :4.100
引用产品:PM150810
-
Melatonin suppresses Akt/mTOR/S6K activity, induces cell apoptosis, and synergistically inhibits cell growth with sunitinib in renal carcinoma cells via reversing Warburg effect(2023/08/29)
作者:Kaihua Xue, Yifan Jiang, Jiyu Bai
期刊:REDOX REPORT
DOI:10.1080/13510002.2023.2251234
影响因子 :3.800
引用产品:PM150810
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Chrysophanol induces apoptosis and ferroptosis of gastric cancer cells by targeted regulation of mTOR(2024/01/07)
作者:Sujuan Xi, Wei Ding, Danping Weng
期刊:Chemical Biology & Drug Design
影响因子 :3.000
引用产品:PM150810
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LINC01268 promotes epithelial-mesenchymal transition, invasion and metastasis of gastric cancer via the PI3K/Akt signaling pathway and targeting MARCKS(2023/08/15)
作者:Linghan Tang, Pengcheng Ye, Lin Yao
期刊:World Journal of Gastrointestinal Oncology
影响因子 :3.000
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Rosiglitazone attenuates high glucose-induced proliferation, inflammation, oxidative stress and extracellular matrix accumulation in mouse mesangial cells through the Gm26917/miR-185-5p pathway(2021/03/30)
作者:Dongbo Zhao, Junli Guo, Lingping Liu
期刊:Endocrine Journal
影响因子 :2.860
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Upregulation of KIF18B facilitates malignant phenotype of esophageal squamous cell carcinoma by activating CDCA8/mTORC1 pathway(2022/09/09)
作者:Jiangliu Xie, Bo Wang, Wenjie Luo
期刊:JOURNAL OF CLINICAL LABORATORY ANALYSIS
影响因子 :2.700
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Comprehensive analysis of COMMD10 as a novel prognostic biomarker for gastric cancer(2023/03/09)
作者:Wenfang Zhao, Jiahui Lin, Sha Cheng
期刊:PeerJ
影响因子 :2.700
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SPI1 Mediates N-Myristoyltransferase 1 to Advance Gastric Cancer Progression via PI3K/AKT/mTOR Pathway(2023/03/17)
作者:Ping Qiu, Xing Li, Min Gong
期刊:Canadian Journal of Gastroenterology and Hepatology
影响因子 :2.700
引用产品:164210 CL-0001 CL-0022 CL-0107 CL-0292 PB180120 PM150110 PM150467 PM150510 PM150810
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Knockdown circZNF131 Inhibits Cell Progression and Glycolysis in Gastric Cancer Through miR-186-5p/PFKFB2 Axis(2022/01/21)
作者:Xingjie Shen, Xiaoyan Zhu, Peixin Hu
期刊:Biochemical Genetics
DOI:10.1007/s10528-021-10165-5
影响因子 :2.400